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Grant support

We thank Prof. Michael N. Hall (Basel) for strains SH100 and SH221, and Prof. Joaquin Arino (Barcelona) for strain BY4741 and its null mutant derivatives fpr1 Delta 0, sit4 Delta 0, and tco89 Delta 0. We also thank Prof. Francisco Portillo (Madrid) for the antibody against the double phosphorylation of Ser911 Thr912 in Pma1. This work was funded by grant PROME-TEOII/2014/041 from Generalitat Valenciana (Valencia, Spain).

Analysis of institutional authors

Dolores Planes, MariaAuthorMiguel Mulet, JoseAuthor

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October 14, 2024
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TOR complex 1 regulates the yeast plasma membrane proton pump and pH and potassium homeostasis

Publicated to:Febs Letters. 591 (13): 1993-2002 - 2017-07-01 591(13), DOI: 10.1002/1873-3468.12673

Authors: Mahmoud, Shima; Dolores Planes, Maria; Cabedo, Marc; Trujillo, Cristina; Rienzo, Alessandro; Caballero-Molada, Marcos; Sharma, Sukesh C; Montesinos, Consuelo; Miguel Mulet, Jose; Serrano, Ramon

Affiliations

Helwan Univ, Fac Farm, Microbiol & Immunol, Qism Helwan, Egypt - Author
Panjab Univ, Dept Biochem, Chandigarh, India - Author
Univ Politecn Valencia, Inst Biol Mol & Celular Plantas, Consejo Super Invest Cient, Edificio 8E,Camino Vera S-N, E-46022 Valencia, Spain - Author

Abstract

We have identified in yeast a connection between two master regulators of cell growth: a biochemical connection involving the TORC1 protein kinase (which activates protein synthesis, nutrient uptake, and anabolism) and a biophysical connection involving the plasma membrane proton-pumping H+-ATPase Pma1 (which drives nutrient and K+ uptake and regulates pH homeostasis). Raising the temperature to nonpermissive values in a TOR thermosensitive mutant decreases Pma1 activity. Rapamycin, a TORC1 inhibitor, inhibits Pma1 dependent on its receptor Fpr1 and on the protein phosphatase Sit4, a TORC1 effector. Mutation of either Sit4 or Tco89, a nonessential subunit of TORC1, decreases proton efflux, K+ uptake, intracellular pH, cell growth, and tolerance to weak organic acids. Tco89 does not affect Pma1 activity but activates K+ transport.

Keywords

Acetic-acidActivatioBiological transportCell membraneCell-growthDirected mutagenesisGrowth-controlH -atpase +H+-atpaseHomeostasisHydrogen-ion concentrationIntracellular phIntracellular spaceK transport +K+ transportMechanistic target of rapamycin complex 1Multiprotein complexesMutationPhPma1 protein, s cerevisiaePotassiumProtein phosphatase 2Proton pumpProton pumpsProton-translocating atpasesRapamycinRestriction pointSaccharomyces cereviciaeSaccharomyces cerevisiaeSaccharomyces cerevisiae proteinsSaccharomyces-cerevisiae genomeSignaling pathwaySitSit4Sit4 protein, s cerevisiaeTco89 protein, s cerevisiaeTorTor serine-threonine kinases

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Febs Letters due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2017, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Biophysics. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 1.75, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jul 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-07-16, the following number of citations:

  • WoS: 17
  • Scopus: 17
  • Europe PMC: 14

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-16:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 45.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 44 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.1.
  • The number of mentions on the social network X (formerly Twitter): 4 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Egypt; India.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Mahmoud, Shima) and Last Author (Serrano, Ramon).

the author responsible for correspondence tasks has been Serrano, Ramon.