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October 28, 2024
Publications
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Article

Gibberellin and abscisic acid transporters facilitate endodermal suberin formation in Arabidopsis

Publicated to: Nature Plants. 9 (5): 785-802 - 2023-05-01 9(5), DOI: 10.1038/s41477-023-01391-3

Authors:

Binenbaum, J; Wulff, N; Camut, L; Kiradjiev, K; Anfang, M; Tal, I; Vasuki, H; Zhang, YQ; Sakvarelidze-Achard, L; Davière, JM; Ripper, D; Carrera, E; Manasherova, E; Ben Yaakov, S; Lazary, S; Hua, CY; Novak, V; Crocoll, C; Weinstain, R; Cohen, H; Ragni, L; Aharoni, A; Band, LR; Achard, P; Nour-Eldin, HH; Shani, E
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Affiliations

Agr Res Org ARO, Inst Plant Sci, Volcani Ctr, Dept Vegetable & Field Crops - Author
CSIC UPV, Inst Biol Mol & Celular Plantas - Author
Tel Aviv Univ, Sch Plant Sci & Food Secur - Author
Univ Copenhagen, Dept Plant & Environm Sci, Plant Nutrients & Food Qual Res Grp - Author
Univ Copenhagen, DynaMo Ctr Excellence, Dept Plant & Environm Sci - Author
Univ Nottingham, Sch Biosci, Div Plant & Crop Sci, Sutton Bonington Campus - Author
Univ Strasbourg, Inst Biol Mol Plantes, CNRS - Author
Univ Tubingen, ZMBP Ctr Plant Mol Biol - Author
Weizmann Inst Sci, Dept Plant & Environm Sci - Author
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Abstract

The authors identified a sub-clade of NPF transporters that orchestrates GA12 long-distance shoot-to-root translocation. Once in the phloem unloading zone, ABA and GA are loaded into pericycle vacuoles and then slowly released to induce endodermal suberin formation in the maturation zone. The plant hormone gibberellin (GA) regulates multiple developmental processes. It accumulates in the root elongating endodermis, but how it moves into this cell file and the significance of this accumulation are unclear. Here we identify three NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER (NPF) transporters required for GA and abscisic acid (ABA) translocation. We demonstrate that NPF2.14 is a subcellular GA/ABA transporter, presumably the first to be identified in plants, facilitating GA and ABA accumulation in the root endodermis to regulate suberization. Further, NPF2.12 and NPF2.13, closely related proteins, are plasma membrane-localized GA and ABA importers that facilitate shoot-to-root GA(12) translocation, regulating endodermal hormone accumulation. This work reveals that GA is required for root suberization and that GA and ABA can act non-antagonistically. We demonstrate how the clade of transporters mediates hormone flow with cell-file-specific vacuolar storage at the phloem unloading zone, and slow release of hormone to induce suberin formation in the maturation zone.
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Keywords

Abscisic acidArabidopsisArabidopsis proteinArabidopsis proteinsCarrier proteinElongationEncodesFamilyGene expression regulationGene expression regulation, plantGeneticsGibberellinGibberellinsGrowthHormoneHormonesMechanismMembrane transport proteinsMetabolismNitrateNitrate transporterNitrate transportersPlant-cellsProteinsSoftwareSuberinTranslocation

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Nature Plants due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2023, it was in position 4/265, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from World Citations provided by WoS (ESI, Clarivate), it yields a value for the citation normalization relative to the expected citation rate of: 7.26. This indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: ESI Nov 13, 2025)

This information is reinforced by other indicators of the same type, which, although dynamic over time and dependent on the set of average global citations at the time of their calculation, consistently position the work at some point among the top 50% most cited in its field:

  • Weighted Average of Normalized Impact by the Scopus agency: 7.31 (source consulted: FECYT Mar 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2026-04-03, the following number of citations:

  • WoS: 35
  • Scopus: 38
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-03:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 78.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 76 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 93.
  • The number of mentions on the social network Facebook: 1 (Altmetric).
  • The number of mentions on the social network X (formerly Twitter): 40 (Altmetric).
  • The number of mentions in news outlets: 10 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Denmark; France; Germany; Israel; United Kingdom.

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