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Analysis of institutional authors

Pastor-Navarro, NAuthorBrun, EmAuthorA. MaquieiraAuthorPuchades, RAuthor

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October 29, 2024
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Development of immunoassays to determinate sulfamethoxazole residues in wastewaters

Publicated to: Journal Of Environmental Monitoring. 11 (5): 1094-1100 - 2009-01-01 11(5), DOI: 10.1039/b818299j

Authors: Pastor-Navarro, Nuria; Brun, Eva M; Gallego-Iglesias, Ester; Maquieira, Angel; Puchades, Rosa

Affiliations

Univ Politecn Valencia, Dept Quim, Inst Quim Mol Aplicada - Author

Abstract

Different immunoassays have been developed to monitor sulfamethoxazole (SMX), an antibiotic frequently detected in water. The immunoassays were developed and optimized in two different formats: ELISA in polystyrene 96-well plate and microarray on compact disc (CD) support. Competitive microimmunoassays were performed by direct adsorption of immunoreagents on the polycarbonate surface of a low-reflectivity CD. Results were displayed using nanogold-labeled immunoglobulins and silver staining developer. High sensitivity was achieved with both formats: LD 0.001 ng mL(-1) in the ELISA-plate, and 0.09 ng mL(-1) in the CD format. The novel CD methodology presents advantages such as simplicity, sensitivity, portability, analytical capacity (2560 spots per disc), in addition to reductions in immunoreagents required, costs and time for analysis. Both proposed methods were applied to determine SMX at nanograms per litre level in wastewater samples without previous preconcentration. Finally, the determination of several fortified wastewater samples using the CD format protocol showed a mean recovery of 87%, which confirms that this assay format is a good screening tool to detect sulfamethoxazole in water samples rapidly and reliably.

Keywords

Analytic methodAnti-infective agentsAntibioticsAntiinfective agentAntimicrobialsAquatic environmentArticleBiosorptionChemical structureChromatographyCompact discsCross reactionDrug residueDrug residuesEffluentsEnzyme linked immunosorbent assayEnzyme-linked immunosorbent assayGoldImmunoassayImmunoglobulinMagnetic resonance spectroscopyMethodologyMicroarray analysisNanoparticleNuclear magnetic resonance spectroscopyPharmaceutical residuesPolycarbonatePolystyrenePriority journalReagentScreeningSensitivity and specificitySilverSilver stainingSpectrophotometry, ultravioletSulfamethoxazoleSulfonamideSulfonamidesSurface propertyTandem mass-spectrometryTimeUltraviolet spectrophotometryWaste waterWaste-waterWater analysisWater pollutantWater pollutants, chemical

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal Of Environmental Monitoring due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2009, it was in position 27/70, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Chemistry, Analytical.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2025-12-05:

  • WoS: 9
  • Scopus: 10
  • Europe PMC: 4

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-12-05:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 11.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 11 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 3.

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Pastor Navarro, Nuria) and Last Author (Puchades Antequera, Rosa Ana).

the author responsible for correspondence tasks has been Maquieira, A.