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Analysis of institutional authors

Simarro, JmsAuthor

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October 31, 2024
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Procedures for ADC Immunoblotting and Immunolocalization for Transmission Electron Microscopy During Organogenic Nodule Formation in Hop

Publicated to: Methods in Molecular Biology. 1694 201-214 - 2018-01-01 1694(), DOI: 10.1007/978-1-4939-7398-9_19

Authors:

Fortes, AM; Simarro, JMS
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Affiliations

Univ Lisbon, Biosyst & Integrat Sci Inst BioISI, Fac Ciencias - Author
Univ Politecn Valencia, Cell Biol Grp COMAV - Author

Abstract

Immunolocalization for transmission electron microscopy is a powerful technique to identify subcellular localization of proteins. This can be combined with molecular and physiological data in order to have a complete overview of protein function. However, optimal sample preservation is required to avoid artefacts. When using chemically fixed samples, the progressive lowering of temperature (PLT) technique is a convenient procedure to dehydrate and embed samples at low temperature, thereby preserving the antigenicity of the proteins to be detected. Despite the advantages of immunogold labelling, it is a time-consuming cell biology technique. Therefore, the quality and specificity of the antibody should be previously checked by western blot. This approach also enables to identify changes in the amount of protein under study throughout development or in response to stress conditions.
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Keywords

Arginine decarboxylaseBiological markerBiomarkersCarboxy-lyasesCarboxylyaseControlled studyElectron microscopyEncapsulationGeneticsHopHumulus lupulusHumulus-lupulusImmunoblottingImmunodetectionImmunogold labelingImmunolocalizationIn vitro studyInternodeMetabolismMicropropagationMicroscopy, electron, transmissionNonhumanOrganogenesisPlant growthPolyacrylamide gel electrophoresisPolyaminesReagentStainingTransmission electron microscopy

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Methods in Molecular Biology, Q3 Agency Scopus (SJR), its regional focus and specialization in Genetics, give it significant recognition in a specific niche of scientific knowledge at an international level.

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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-03:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 1.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 1 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 12.
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Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Portugal.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: Last Author (Tiburcio, AF).

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