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Analysis of institutional authors

Murguia, JrAuthor

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October 31, 2024
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A novel role for protein kinase Gcn2 in yeast tolerance to intracellular acid stress

Publicated to: BIOCHEMICAL JOURNAL. 441 (0): 255-264 - 2012-01-01 441(0), DOI: 10.1042/BJ20111264

Authors:

Hueso, G.; APARICIO-SANCHIS, R.; Montesinos, C.; LORENZ, S.; Murguía, J.R.; Serrano, R.
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Affiliations

Univ Politecn Valencia CSIC, Inst Biol Mol & Celular Plantas - Author

Abstract

Intracellular pH conditions many cellular systems, but its mechanisms of regulation and perception are mostly unknown. We have identified two yeast genes important for tolerance to intracellular acidification caused by weak permeable acids. One corresponded to LEU2 and functions by removing the dependency of the leu2 mutant host strain on uptake of extracellular leucine. Leucine transport is inhibited by intracellular acidification, and either leucine oversupplementation or overexpression of the transporter gene BAP2 improved acid growth. Another acid-tolerance gene is GCN2, encoding a protein kinase activated by uncharged tRNAs during amino acid starvation. Gcn2 phosphorylates eIF2 alpha (eukaryotic initiation factor 2 alpha) (Sui2) at Ser(51) and this inhibits general translation, but activates that of Gcn4, a transcription factor for amino acid biosynthetic genes. Intracellular acidification activates Gcn2 probably by inhibition of aminoacyl-tRNA synthetases because we observed accumulation of uncharged tRNA(leu) without leucine depletion. Gcn2 is required for leucine transport and a gcn2-null mutant is sensitive to acid stress if auxotrophic for leucine. Gcn4 is required for neither leucine transport nor acid tolerance, but a S51A sui2 mutant is acid-sensitive. This suggests that Gcn2, by phosphorylating eIF2 alpha, may activate translation of an unknown regulator of amino acid transporters different from Gcn4.
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Keywords

Acetic acidAcetic-acidAcid toleranceAcidificationAcidityAdaptation, physiologicalAmino acid transportAmino acidsAmino acyl-trna synthetasesAminoacyl transfer rnaArticleAuxotrophic mutantControlled studyFungal geneFungal strainFungus growthGcn2Gcn2 protein, s cerevisiaeGene activationGene expression regulation, fungalGene functionGene identificationGene overexpressionGene-expressionGenomeH+-atpaseHomeostasisHydrogen-ion concentrationInitiation factor 2alphaLeucineMaster regulatorMutationNonhumanPhPh homoeostasisPlasma-membrane atpasePlasmidsPriority journalProtein kinaseProtein kinase ggn2Protein malnutritionProtein phosphorylationProtein serine-threonine kinasesProtein-serine-threonine kinasesRna, fungalRna, transferSaccharomyces cerevisiaeSaccharomyces cerevisiae proteinsSaccharomyces-cerevisiaeSalt toleranceSerineSignal transductionStress, physiologicalTransfer rnaTransfer-rnaUnclassified drug

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal BIOCHEMICAL JOURNAL due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2012, it was in position 61/290, thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry & Molecular Biology.

Independientemente del impacto esperado determinado por el canal de difusión, es importante destacar el impacto real observado de la propia aportación.

Según las diferentes agencias de indexación, el número de citas acumuladas por esta publicación hasta la fecha 2026-04-02:

  • WoS: 36
  • Scopus: 40
  • Europe PMC: 30
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Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2026-04-02:

  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 72 (PlumX).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.
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Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Hueso, G) and Last Author (Serrano, R).

the author responsible for correspondence tasks has been Serrano, R.

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