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Analysis of institutional authors

Coronado-Martín, AAuthorJáquez, MAuthorAtarés, ACorresponding Author

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October 11, 2024
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Micropropagation and genetic transformation of Byblis liniflora

Publicated to:In Vitro Cellular & Developmental Biology-Plant. 60 (5): 634-645 - 2024-08-06 60(5), DOI: 10.1007/s11627-024-10448-7

Authors: Coronado-Martin, Alberto; Martin-Vasquez, Constanza; Jaquez, Marybel; Bahaji, Abdellatif; Atares, Alejandro

Affiliations

CSIC Gobierno Navarra, Inst Agrobiotecnol IdAB, Irunako Etorbidea 123 - Author
Univ Politecn Valencia, Inst Plant Mol & Cellular Biol IBMCP, Camino Vera Sn - Author

Abstract

Byblis, a small genus of carnivorous plants predominantly found in Australia, is characterized by its passive trapping mechanism and unique floral features. The chemical composition of Byblis, including identified phenylethanoid glycosides, particularly acteoside, highlights its pharmacological potential with various biological activities. In vitro culture techniques have been established for propagation, with micropropagation protocols developed for different Byblis species. However, information on genetic transformation, vital for trait modification and enhanced pharmacological interest, remains limited. This study focuses on optimizing micropropagation, adventitious regeneration, and genetic transformation methods for Byblis liniflora. Adventitious regeneration rates were highest in medium with reduced Murashige and Skoog salts (MS/10) and sucrose (3 gL(-1)) concentrations. Zeatin supplementation (1 mgL(-1)) further improved regeneration rates and bud development with 100% of regenerated root explants and 8.8 shoots per explant. Liquid MB3 medium supplemented with indole-3-acetic acid (IAA) 5 mgL(-1) facilitated efficient rooting and acclimatization. The establishment of an efficient Rhizobium-mediated genetic transformation method yielded transgenic plants expressing green fluorescent protein (GFP). Molecular analysis confirmed transgene integration, marking the first successful genetic transformation in the Byblis genus. These advancements pave the way for exploring gene function and enhancing pharmacological properties, thereby broadening our understanding and utilization of carnivorous plants like Byblis.

Keywords

AcclimatizationCarnivorous plantCarnivorous plantsDroseraGrowthIn vitro multiplicationIn-vitro regenerationPhenylethanoid glycosidesPropagationRainbow plantSundewTransgenicTrap

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal In Vitro Cellular & Developmental Biology-Plant due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2024 there are still no calculated indicators, but in 2023, it was in position 166/205, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Cell Biology. Notably, the journal is positioned en el Cuartil Q2 para la agencia Scopus (SJR) en la categoría Plant Science.

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-07-07:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 2.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 5 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 3.1.
  • The number of mentions on the social network X (formerly Twitter): 6 (Altmetric).

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Coronado Martín, Alberto) and Last Author (Atarés Huerta, Alejandro).

the author responsible for correspondence tasks has been Atarés Huerta, Alejandro.