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Analysis of institutional authors

March, CAuthorManclús, JjAuthorAbad, AAuthorNavarro, AAuthor

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Rapid detection and counting of viable beer-spoilage lactic acid bacteria using a monoclonal chemiluminescence enzyme immunoassay and a CCD camera

Publicated to:Journal Of Immunological Methods. 303 (1-2): 92-104 - 2005-08-01 303(1-2), DOI: 10.1016/j.jim.2005.06.002

Authors: March, C; Manclús, JJ; Abad, A; Navarro, A; Montoya, A

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Abstract

A chemiluminescence enzyme immunoassay carried out with a monoclonal antibody (MAb) and a charge-coupled device (CCD) camera was developed for rapid enumeration of viable beer-spoilage lactic acid bacteria. LA-4 Mab, which recognizes a broad spectrum of lactic acid bacteria isolated from several breweries across Spain, was produced and characterized. Test samples were filtered through polycarbonate membranes, and the membranes with retained bacteria were incubated at 31 degrees C for 2 days. They were then subjected to a two-step chemiluminescence enzyme immunoassay with MAb LA-4, and light-emitting points were detected and counted with a CCD camera. Eighteen out of 19 beer-spoilage lactic acid bacteria analysed produced luminous spots that could be enumerated. Results provided by the immunochemiluminescence assay correlated very well with those obtained by visual plate counting within a range of 3-100 CFU/100 ml. Correlation coefficients were 0.994 for four strains in sterile saline solution and 0.984 for 14 strains in artificially contaminated beer. The excellent agreement suggests that luminous spots detected within 2 days of culture are produced only by viable cells. (c) 2005 Elsevier B.V. All rights reserved.

Keywords

Animal cellAnimalsAntibodiesAntibodies, monoclonalArticleBacterial cellBacterial countBacterial strainBacterium contaminationBacterium detectionBacterium isolationBeerBeer-spoilage bacteriaBioluminescenceCameraCcd camerCcd cameraCell viabilityChemiluminescent measurementsChemoluminescenceColony count, microbialControlled studyCorrelation analysisCorrelation coefficientEnzyme immunoassayEnzyme-linked immunosorbent assayEscherichia-coliFemaleFood contaminationFood spoilageImmunoassayImmunochemiluminescenceLactic acid bacteriumLactobacillusLuminanceLuminescent measurementsMiceMice, inbred balb cMonoclonal antibodyMouseNonhumanPolycarbonatePolymerase-chain-reactionPriority journalProbesProteinSaccharomyces cerevisiaeSalmonellaSignal processing, computer-assistedSodium chlorideSpainTv cameraVideo recordingWate

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Journal Of Immunological Methods due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2005, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Immunology and Allergy.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 1.71, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-18, the following number of citations:

  • WoS: 34
  • Scopus: 40
  • Europe PMC: 11
  • OpenCitations: 37

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-18:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 33.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 33 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 3.

Leadership analysis of institutional authors

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (March Moya, Carlos) and Last Author (Montoya, A).

the author responsible for correspondence tasks has been Montoya, A.