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Analysis of institutional authors

Lozano-Juste, JAuthorNebauer, SgAuthor

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October 30, 2024
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PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue

Publicated to:Cells. 11 (5): 795- - 2022-03-01 11(5), DOI: 10.3390/cells11050795

Authors: Pizzio, Gaston A; Mayordomo, Cristian; Lozano-Juste, Jorge; Garcia-Carpintero, Victor; Vazquez-Vilar, Marta; Nebauer, Sergio G; Kaminski, Kacper P; Ivanov, Nikolai V; Estevez, Juan C; Rivera-Moreno, Maria; Albert, Armando; Orzaez, Diego; Rodriguez, Pedro L

Affiliations

CSIC, Inst Quim Fis Rocasolano, Dept Cristalog & Biol Estruct - Author
Philip Morris Prod SA, PMI R&D, Quai Jean Renaud 5 - Author
Univ Politecn Valencia, Inst Biol Mol & Celular Plantas, CSIC - Author
Univ Politecn Valencia, Plant Prod Dept - Author
Univ Santiago de Compostela, Ctr Singular Invest Quim & Biol Mol CiQUS, Dept Quim Organ - Author
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Abstract

To face the challenges of climate change and sustainable food production, it is essential to develop crop genome editing techniques to pinpoint key genes involved in abiotic stress signaling. The identification of those prevailing abscisic acid (ABA) receptors that mediate plant-environment interactions is quite challenging in polyploid plants because of the high number of genes in the PYR/PYL/RCAR ABA receptor family. Nicotiana benthamiana is a biotechnological crop amenable to genome editing, and given the importance of ABA signaling in coping with drought stress, we initiated the analysis of its 23-member family of ABA receptors through multiplex CRISPR/Cas9-mediated editing. We generated several high-order mutants impaired in NbPYL1-like and NbPYL8-like receptors, which showed certain insensitivity to ABA for inhibition of seedling establishment, growth, and development of shoot and lateral roots as well as reduced sensitivity to the PYL1-agonist cyanabactin (CB). However, in these high-order mutants, regulation of transpiration was not affected and was responsive to ABA treatment. This reveals a robust and redundant control of transpiration in this allotetraploid plant that probably reflects its origin from the extreme habitat of central Australia.

Keywords

Aba receptorsAba sensitivityAbiotic stressAbscisic acidAbscisic-acid receptorsAmino acid sequenceArabidopsisArabidopsis proteinArabidopsis proteinsArticleBiochemical analysisBiotechnological cropCarrier proteinCarrier proteinsCas9Climate changeControlled studyCoping behaviorCrisprCrispr-cas9 systemCrispr/cas9Crystal structureDroughtDrought stressEscherichia coliExpressionExtremophileGel filtration chromatographyGene editingGene expressionGene expression regulationGene expression regulation, plantGene mutationGenetic analysisGenetic transformationGeneticsGenomic dnaIc50MechanismMetabolismMultiplex mutationsMutational analysisNicotianaNicotiana benthamianaNonhumanPhenotypePhylogenyPlant growthPlant immunityPlant seedPolyacrylamide gel electrophoresisPolymerase chain reactionPolyploidyProtein phosphorylationProtein purificationProtein qualityPyrabactin resistance like 1Pyrabactin resistance like 8Recombinant proteinRna sequenceRoot growthSeedsSensitivity analysisSequence alignmentShoot growthSignal transductionSite directed mutagenesisStomatal apertureTetraploidTetraploidyTobaccoTransgenic plantUnclassified drugUpregulation

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Cells due to its progression and the good impact it has achieved in recent years, according to the agency Scopus (SJR), it has become a reference in its field. In the year of publication of the work, 2022, it was in position , thus managing to position itself as a Q1 (Primer Cuartil), in the category Biochemistry, Genetics and Molecular Biology (Miscellaneous).

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 2.92, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Aug 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-08-13, the following number of citations:

  • WoS: 7
  • Scopus: 8
  • Europe PMC: 4

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-08-13:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 15.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 15 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 2.
  • The number of mentions on the social network X (formerly Twitter): 3 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Switzerland.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Pizzio, GA) and Last Author (Rodriguez, PL).

the author responsible for correspondence tasks has been Rodriguez, PL.