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Analysis of institutional authors

Rodriguez, AAuthor

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Engineering D-limonene synthase down-regulation in orange fruit induces resistance against the fungus Phyllosticta citricarpa through enhanced accumulation of monoterpene alcohols and activation of defence

Publicated to:Molecular Plant Pathology. 19 (9): 2077-2093 - 2018-09-01 19(9), DOI: 10.1111/mpp.12681

Authors: Rodríguez, A; Kava, V; Latorre-García, L; da Silva, GJ ; Pereira, RG; Glienke, C; Ferreira-Maba, LS; Vicent, A; Shimada, T; Peña, L

Affiliations

Consejo Super Invest Cient IBMCP CSIC, Inst Biol Mol & Celular Plantas - Author
Fundo Defesa Citricultura Fundecitrus, Lab Biotecnol Vegetal, BR-14807040 Araraquara - Author
Inst Valenciano Invest Agr IVIA, Ctr Protecc Vegetal & Biotecnol - Author
Natl Agr & Biooriented Res Org NARO, Natl Inst Fruit Tree Sci NIFTS, Shizuoka - Author
Univ Fed Parana, Dept Genet, BR-81531980 Curitiba - Author
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Abstract

Terpene volatiles play an important role in the interactions between specialized pathogens and fruits. Citrus black spot (CBS), caused by the fungus Phyllosticta citricarpa, is associated with crop losses in different citrus-growing areas worldwide. The pathogen may infect the fruit for 20-24weeks after petal fall, but the typical hard spot symptoms appear when the fruit have almost reached maturity, caused by fungal colonization and the induction of cell lysis around essential oil cavities. D-Limonene represents approximately 95% of the total oil gland content in mature orange fruit. Herein, we investigated whether orange fruit with reduced d-limonene content in peel oil glands via an antisense (AS) approach may affect fruit interaction with P.citricarpa relative to empty vector (EV) controls. AS fruit showed enhanced resistance to the fungus relative to EV fruit. Because of the reduced d-limonene content, an over-accumulation of linalool and other monoterpene alcohols was found in AS relative to EV fruit. A global gene expression analysis at 2 h and 8 days after inoculation with P.citricarpa revealed the activation of defence responses in AS fruit via the up-regulation of different pathogenesis-related (PR) protein genes, probably as a result of enhanced constitutive accumulation of linalool and other alcohols. When assayed invitro and invivo, monoterpene alcohols at the concentrations present in AS fruit showed strong antifungal activity. We show here that terpene engineering in fruit peels could be a promising method for the development of new strategies to obtain resistance to fruit diseases.

Keywords

BiosynthesisCbsCitrus black spotCitrus sinensisD-limoneneDispersalEssential oilsExpressionFruitGenetic engineeringGuignardia citricarpaGuignardia-citricarpaIntramolecular lyasesIsomeraseLinaloolLinalool confers resistanceMetabolismMicrobiologyMonoterpenesPhyllosticta citricarpaPinene cyclase iProceduresResponsesSubsp citriSweet orangeTerpeneValencia oranges

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Molecular Plant Pathology due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2018, it was in position 15/228, thus managing to position itself as a Q1 (Primer Cuartil), in the category Plant Sciences. Notably, the journal is positioned above the 90th percentile.

From a relative perspective, and based on the normalized impact indicator calculated from the Field Citation Ratio (FCR) of the Dimensions source, it yields a value of: 2.57, which indicates that, compared to works in the same discipline and in the same year of publication, it ranks as a work cited above average. (source consulted: Dimensions Jun 2025)

Specifically, and according to different indexing agencies, this work has accumulated citations as of 2025-06-19, the following number of citations:

  • WoS: 17
  • Scopus: 18
  • OpenCitations: 16

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-19:

  • The use, from an academic perspective evidenced by the Altmetric agency indicator referring to aggregations made by the personal bibliographic manager Mendeley, gives us a total of: 56.
  • The use of this contribution in bookmarks, code forks, additions to favorite lists for recurrent reading, as well as general views, indicates that someone is using the publication as a basis for their current work. This may be a notable indicator of future more formal and academic citations. This claim is supported by the result of the "Capture" indicator, which yields a total of: 56 (PlumX).

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

  • The Total Score from Altmetric: 4.
  • The number of mentions on the social network X (formerly Twitter): 9 (Altmetric).

It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

  • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

Leadership analysis of institutional authors

This work has been carried out with international collaboration, specifically with researchers from: Brazil; Japan.

There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (Rodríguez León, Alejandro) and Last Author (Pena, L).

the author responsible for correspondence tasks has been Pena, L.